biorad chemidoc imaging station (Bio-Rad)
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biorad chemidoc imaging station
Biorad Chemidoc Imaging Station, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 34698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+chemidoc+imaging+station/ChemiDoc+Imaging+System/pm38850255-97-11-11
Average 99 stars, based on 34698 article reviews
Biorad Chemidoc Imaging Station, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 34698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biorad+chemidoc+imaging+station/ChemiDoc+Imaging+System/pm38850255-97-11-11
Average 99 stars, based on 34698 article reviews
biorad chemidoc imaging station - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: Complement 3 + -astrocytes are highly abundant in prion diseases, but their abolishment led to an accelerated disease course and early dysregulation of microglia. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to nitrocellulose membranes (0.2 μm pore size, BioRad) at 400 mA for 1 h, blocked for 1 h at room temperature in 5% milk powder in TBST buffer and incubated overnight at 4 °C with anti-PrP antibody Pom1 [50], Iba1 (Wako), Actin (Millipore), and GLP-1R (Santa Cruz) [63]. .. After washing and incubation for 1 h at room temperature with an HRPconjugated anti-mouse or anti-rabbit secondary antibody (1:10.000 in blocking buffer), signals were detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Muskelin Coordinates PrP C Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Article Snippet: Samples in sample buffer were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to PVDF membranes (BioRad) at 400 mA for 1 hr, blocked for 1 hr at room temperature in 5% milk/TBST and incubated overnight at 4 C with anti-PrP antibody POM2 (1:1,000) and Tsg101, as loading control (1:1,000; BD PharMingen or GeneTex). .. After incubation for 1 h at room temperature with an HRP-conjugated secondary antibody (1:5,000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Muskelin Coordinates PrP C Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to PVDF membranes (BioRad) at 400 mA for 1 hr, blocked for 1 h at room temperature in 5% milk/TBST and incubated overnight at 4 C with anti-PrP antibody POM1 (1:1000; Polymenidou et al., 2008) in blocking buffer or b-actin (1:5,000; Sigma). .. After incubation for 1 h at room temperature with an HRP-conjugated secondary antibody (1:5000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Activation of microglia by retroviral infection correlates with transient clearance of prions from the brain but does not change incubation time. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules), transferred to PVDF membranes (BioRad) at 400 mA for 1 h, blocked for 1 h at room temperature in proteinfree blocking buffer (Thermo Scientific, Rockfort) and incubated overnight at 48C with anti-PrP antibody Pom1 (1:1000 in blocking buffer; provided by Adriano Aguzzi, Z€urich) (43). .. After incubation for 1 h at room temperature with an HRP-conjugated anti-mouse secondary antibody (1:5000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Blocking Assay:Article Title: Complement 3 + -astrocytes are highly abundant in prion diseases, but their abolishment led to an accelerated disease course and early dysregulation of microglia. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to nitrocellulose membranes (0.2 μm pore size, BioRad) at 400 mA for 1 h, blocked for 1 h at room temperature in 5% milk powder in TBST buffer and incubated overnight at 4 °C with anti-PrP antibody Pom1 [50], Iba1 (Wako), Actin (Millipore), and GLP-1R (Santa Cruz) [63]. .. After washing and incubation for 1 h at room temperature with an HRPconjugated anti-mouse or anti-rabbit secondary antibody (1:10.000 in blocking buffer), signals were detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Muskelin Coordinates PrP C Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Article Snippet: Samples in sample buffer were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to PVDF membranes (BioRad) at 400 mA for 1 hr, blocked for 1 hr at room temperature in 5% milk/TBST and incubated overnight at 4 C with anti-PrP antibody POM2 (1:1,000) and Tsg101, as loading control (1:1,000; BD PharMingen or GeneTex). .. After incubation for 1 h at room temperature with an HRP-conjugated secondary antibody (1:5,000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Muskelin Coordinates PrP C Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to PVDF membranes (BioRad) at 400 mA for 1 hr, blocked for 1 h at room temperature in 5% milk/TBST and incubated overnight at 4 C with anti-PrP antibody POM1 (1:1000; Polymenidou et al., 2008) in blocking buffer or b-actin (1:5,000; Sigma). .. After incubation for 1 h at room temperature with an HRP-conjugated secondary antibody (1:5000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Activation of microglia by retroviral infection correlates with transient clearance of prions from the brain but does not change incubation time. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules), transferred to PVDF membranes (BioRad) at 400 mA for 1 h, blocked for 1 h at room temperature in proteinfree blocking buffer (Thermo Scientific, Rockfort) and incubated overnight at 48C with anti-PrP antibody Pom1 (1:1000 in blocking buffer; provided by Adriano Aguzzi, Z€urich) (43). .. After incubation for 1 h at room temperature with an HRP-conjugated anti-mouse secondary antibody (1:5000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Imaging:Article Title: Complement 3 + -astrocytes are highly abundant in prion diseases, but their abolishment led to an accelerated disease course and early dysregulation of microglia. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to nitrocellulose membranes (0.2 μm pore size, BioRad) at 400 mA for 1 h, blocked for 1 h at room temperature in 5% milk powder in TBST buffer and incubated overnight at 4 °C with anti-PrP antibody Pom1 [50], Iba1 (Wako), Actin (Millipore), and GLP-1R (Santa Cruz) [63]. .. After washing and incubation for 1 h at room temperature with an HRPconjugated anti-mouse or anti-rabbit secondary antibody (1:10.000 in blocking buffer), signals were detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Muskelin Coordinates PrP C Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Article Snippet: Samples in sample buffer were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to PVDF membranes (BioRad) at 400 mA for 1 hr, blocked for 1 hr at room temperature in 5% milk/TBST and incubated overnight at 4 C with anti-PrP antibody POM2 (1:1,000) and Tsg101, as loading control (1:1,000; BD PharMingen or GeneTex). .. After incubation for 1 h at room temperature with an HRP-conjugated secondary antibody (1:5,000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Muskelin Coordinates PrP C Lysosome versus Exosome Targeting and Impacts Prion Disease Progression. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules, USA), transferred to PVDF membranes (BioRad) at 400 mA for 1 hr, blocked for 1 h at room temperature in 5% milk/TBST and incubated overnight at 4 C with anti-PrP antibody POM1 (1:1000; Polymenidou et al., 2008) in blocking buffer or b-actin (1:5,000; Sigma). .. After incubation for 1 h at room temperature with an HRP-conjugated secondary antibody (1:5000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a Article Title: Glycan-Tailored Glycoproteomic Analysis Reveals Serine is the Sole Residue Subjected to O -Linked Glycosylation in Acinetobacter baumannii . Article Snippet: Protein glycosylation is a ubiquitous process observed across all domains of life.. Within the human pathogen Acinetobacter baumannii, Olinked glycosylation is required for virulence; however, the targets and conservation of glycosylation events remain poorly defined.. In this work, we expand our understanding of the breadth and site specificity of glycosylation within A. baumannii by demonstrating the value of strain specific glycan electron-transfer/higher-energy collision dissociation (EThcD) triggering for bacterial glycoproteomics. Article Title: Activation of microglia by retroviral infection correlates with transient clearance of prions from the brain but does not change incubation time. Article Snippet: Samples were analyzed by SDS-page (AnykD, Biorad, Hercules), transferred to PVDF membranes (BioRad) at 400 mA for 1 h, blocked for 1 h at room temperature in proteinfree blocking buffer (Thermo Scientific, Rockfort) and incubated overnight at 48C with anti-PrP antibody Pom1 (1:1000 in blocking buffer; provided by Adriano Aguzzi, Z€urich) (43). .. After incubation for 1 h at room temperature with an HRP-conjugated anti-mouse secondary antibody (1:5000 in blocking buffer), signal was detected with ECL femto reagent (Thermo Scientific) and visualized and quantified with a |